Context: The forced swim test (FST) is employed to examine depression and depressive-like behaviors in rodents, such as mice and rats. In this test, increased periods of immobility and decreased swimming by the animal indicate heightened despair and depression-like behaviors. Evidence Acquisition: This review discusses the impacts of the animals' race, gender, age, and weight and environmental factors like light, noise, and smell on the FST. Results: Our review reveals that racial differences in rats and mice can influence their behavior. Differences in the nervous system structure and sex hormones related to gender are also significant. Additionally, animals that are very young or old, and those that are either very overweight or underweight, are unsuitable for the FST. Environmental factors such as light, noise, and smell were identified as confounding factors that could influence the outcomes and compromise the study's reliability. Conclusions: It is essential to consider these factors and enhance the conditions and environment to carry out a standardized test. Furthermore, by acquiring more detailed information about these factors and minimizing or eliminating their effects, studies can yield more reliable results.